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RAPTOR antibody | 1A9

Mouse anti RAPTOR

Product Type
PrecisionAb Monoclonal
Clone
1A9
Isotype
IgG1
Format
Purified
Specificity
RAPTOR

Product Code Applications Pack Size List Price Your Price Qty
VMA00311
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human RAPTOR antibody recognizes the regulatory-associated protein of mTOR (RAPTOR), also known as p150 target of rapamycin (TOR)-scaffold protein.

Encoded by the RPTOR gene, regulatory-associated protein of mTOR is a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. RAPTOR forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for RPTOR (provided by RefSeq, Sep 2009).

Mouse anti Human RAPTOR antibody detects a band of 149 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti RAPTOR detects a band of approximately 149 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant RAPTOR
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q8N122
Entrez Gene
RPTOR
GO Terms
GO:0016049 cell growth
GO:0005764 lysosome
GO:0005829 cytosol
GO:0007050 cell cycle arrest
GO:0008286 insulin receptor signaling pathway
GO:0031669 cellular response to nutrient levels
GO:0031929 TOR signaling cascade
GO:0031931 TORC1 complex
GO:0032008 positive regulation of TOR signaling cascade
GO:0032403 protein complex binding
GO:0071230 cellular response to amino acid stimulus
GO:0071902 positive regulation of protein serine/threonine kinase activity

VMA00311

151204

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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