RAP1A/B Antibody (PrecisionAbTM Antibody) | AB01/2C6.10.4

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RAP1A/B Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB01/2C6.10.4 gallery image 1

Western blot analysis of whole cell lysates probed with RAP1A/B antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to RAP1A/B (molecular weight 21 kDa)

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  • RAP1A/B Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB01/2C6.10.4 thumbnail image 1
  • Mouse anti RAP1A/B
  • RAP1A/B Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    PrecisionAb™ Monoclonal
  • Isotype
    IgG2b
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VMA00546WBdatasheet pdfdatasheet pdf100 µl
    VMA00546
    VMA00546KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VMA00546KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
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    • Mouse anti Human RAP1A/B antibody recognizes the Ras-related protein Rap-1b, also known as GTP-binding protein smg p21B, Ras family small GTP binding protein RAP1B or small GTP binding protein.

      RAP1B gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of RAP1B are found on chromosomes 3, 5, 6 and 9 (provided by RefSeq, Oct 2011).

      Mouse anti Human RAP1A/B antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Anti RAP1A/B detects a band of approximately 21 kDa in K562 cell lysates
      Anti RAP1A/B detects a band of approximately 21 kDa in K562 cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
      20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      E. coli-derived recombinant human RAP1A/B (amino acids 1 - 184)
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg K562 lysate lyophilized in RIPA buffer
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      GTP binding
      blood coagulation
      plasma membrane
      energy reserve metabolic process
      activation of MAPKK activity
      protein binding
      nerve growth factor receptor signaling pathway
      GTPase activity
      GDP binding
      cytosol
      regulation of insulin secretion
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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