RANTES (regulated upon activation, normal T cell expressed and secreted), otherwise known as CCL5, is a 68 amino acid CC- chemokine which acts as a chemoattractant for monocytes, CD4+/CD45RO+ memory T cells, basophils and eosinophils, thorough binding to chemokine (C-C motif) receptor 1 (CCR1), CCR3 and CCR5.
Purified recombinant protein - lyophilised
Reconstitute with 0.2ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant RANTES expressed in E. coli.
>98% by SDS PAGE
Approx. Protein Concentrations
Total protein concentration 0.1mg/ml after reconstitution
Protein Molecular Weight
7.8kD (68 Amino acid sequence)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate positive/negative controls.