RAGE Antibody (PrecisionAbTM Antibody)

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RAGE Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with RAGE antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 6 second exposure. Arrow points to RAGE (molecular weight 55 kDa)

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  • Rabbit anti RAGE
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    Rabbit anti-human RAGE antibody recognizes advanced glycosylation end product-specific receptor, also known as RAGE isoform NtRAGE-delta, RAGE isoform sRAGE-delta or receptor for advanced glycation end-products variant 20. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00582WBdatasheet pdfdatasheet pdf100 µl
      VPA00582
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human RAGE antibody recognizes RAGE also known as advanced glycosylation end product-specific receptor, RAGE isoform NtRAGE-delta, AGER or receptor for advanced glycation end-products variant 20.

        The advanced glycosylation end product (AGE) receptor encoded by the AGER gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for AGER (Hudson et al. 2008) (provided by RefSeq, May 2011).

        Rabbit anti Human RAGE antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using tissue and whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti RAGE detects a band of approximately 55 kDa in mouse lung tissue
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
      • Preparation
        Rabbit polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        2% Sucrose
      • Immunogen
        Synthetic peptide directed towards the N terminal region of human RAGE
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        neuron projection development
        integral to plasma membrane
        S100 alpha binding
        inflammatory response
        cell surface receptor linked signaling pathway
        innate immune response
        transmembrane receptor activity
        positive regulation of NF-kappaB transcription factor activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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