RAGE Antibody

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RAGE Antibody gallery image 1

Immunoperoxidase staining of formalin fixed, paraffin embedded porcine small intestine with Goat anti Human RAGE (AHP594), bottom with no-antibody control, top
Image courtesy of Prof. I. Splichal, Academy of Sciences of the Czech Republic, Novy Hradek

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RAGE Antibody gallery image 2

Immunoperoxidase staining of formalin fixed, paraffin embedded porcine lung with Goat anti Human RAGE (AHP594), bottom with no-antibody control, top
Image courtesy of Prof. I. Spichal, Academy of Sciences of the Czech Republic, Novy Hradek

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RAGE Antibody gallery image 3

Published customer image:
FPR1 and FPRL1 are co-localised with RAGE in primary rat glial cells. Astrocytes and microglial cells were fixed and labelled with anti-FPR1 (A) or anti-FPRL1 (B) and anti-RAGE antibodies. Localisation of FPR1, FPRL1 and RAGE was examined by double fluorescence microscopy. Bisbenzimide was used for nuclear counter-staining (blue). The figures show representative results from one of three independent experiments, each performed in duplicate. Scale bar: 20?μm. The right columns showed resulting scatter plot of intensities across the two channels. The Pearson correlation coefficient rp and Spearman correlation coefficient rs are indicated on the scatter plots. For FPR1 (green channel) and RAGE (red channel) (A), low co-localisation and for FPRL1 (green channel) and RAGE (red channel) (B) good co-localisation.

From: Slowik A, Merres J, Elfgen A, Jansen S, Mohr F, Wruck CJ, Pufe T, Brandenburg LO. Involvement of formyl peptide receptors in receptor for advanced glycation end products (RAGE)--and amyloid beta 1-42-induced signal transduction in glial cells. Mol Neurodegener. 2012 Nov 20;7:55.

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RAGE Antibody gallery image 4

Published customer image:
FPR1 and FPRL1 are co-localised with RAGE in transfected HEK293 cells. FPR1 (A) or FPRL1 (B) and RAGE or ΔRAGE transfected HEK293 cells were fixed and labelled with anti-FPR1 (A) or anti-FPRL1 (B) and anti-RAGE antibodies. Localisation of FPR1, FPRL1 and RAGE/ΔRAGE was examined by double fluorescence microscopy. Bisbenzimide was used for nuclear counter-staining (blue). The figures show representative results from one of three independent experiments, each performed in duplicate. Scale bar: 20 μm. The right columns show the resulting scatter plot of intensities across the two channels. The Pearson correlation coefficient rp and Spearman correlation coefficient rs are indicated on the scatter plots. For FPR1/FPRL1 (green channel) and RAGE/ΔRAGE (red channel), please note a good co-localisation.

From: Slowik A, Merres J, Elfgen A, Jansen S, Mohr F, Wruck CJ, Pufe T, Brandenburg LO. Involvement of formyl peptide receptors in receptor for advanced glycation end products (RAGE)--and amyloid beta 1-42-induced signal transduction in glial cells. Mol Neurodegener. 2012 Nov 20;7:55.

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  • Goat anti Human RAGE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP594E, FN*, P *, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP594
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Human RAGE antibody recognises the human Advanced glycosylation end product-specific receptor, also known as Receptor for Advanced Glycation End products (RAGE). RAGE is a 404 amino acid ~45 kDa single pass type 1 transmembrane glycoprotein bearing two Ig-like C2-type and a single Ig-like V-type domain. RAGE is a member of the Immunoglobulin superfamily which binds a variety of ligands including advanced glycation end products (AGEs) and amyloid fibrils. RAGE is expressed by endothelium, mononuclear phagocytes, smooth muscle and neurons. RAGE may be involved in a range of pathological conditions including diabetes (Ndip et al. 2014) and Alzheimer's disease (Matrone et al. 2014).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      BovineExpected from Sequence
      ChimpanzeeExpected from Sequence
      Dogyes
      Pigyes
      RabbitExpected from Sequence
      Rhesus MonkeyExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to Human RAGE were raised by repeated immunisation of goats with highly purified antigen.
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide, PKKPPQRLEWKLNTGRTE, corresponding to amino acids 42-59 of human RAGE.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      S100 alpha binding
      inflammatory response
      integral to plasma membrane
      positive regulation of NF-kappaB transcription factor activity
      transmembrane receptor activity
      neuron projection development
      innate immune response
      cell surface receptor linked signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/250K1/270K
      Western Blotting1/3000
      Functional Assays(1)
      Immunohistology - Paraffin(2)1/400
      (1)
      This product has been reported to have use in functional assays (Rodríguez-Ayala) however the product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose.
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Diabetic kidney
    • Immunofluorescence
    • Western Blotting
      AHP594 detects a band of approximately 45 kDa in mouse and rat lung lysates.
    • Instructions For Use

    Additional RAGE Antibody Formats

    Formats Applications Sizes available
    RAGE Antibody : Serum E, FN*, P *, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Diabetic kidney
              • Application NameReference Images
                Functional Assays
                Immunofluorescence

              References

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