Radixin Antibody

Radixin Antibody gallery image 1

Western blotting analysis of human tonsil lysate (35μg protein in RIPA buffer) probed using Goat anti Human radixin antibody (AHP1401) at 0.03μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Radixin Antibody gallery image 2

Western blotting analysis of NIH3T3 (A), HeLa (B), Jurkat (C), and A431 (D) cell line lysates (35μg protein in RIPA buffer) probed with Goat anti Human radixin antibody (AHP1401). Primary incubation was 1 hour. Detected by chemiluminescence.

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  • Goat anti Human Radixin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1401E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human Radixin antibody recognizes human radixin, a 583 amino acid inner leaf cell membrane protein bearing a single FERM domain, a member of the ezrin, radixin, moesin (ERM) protein family. Radixin is involved in cytoskeletal anchoring playing a crucial role as a linker protein between the plasma membrane and the barbed end of the actin cytoskeleton (Salles et al. 2013).

      Radixin shares high sequence homology with both ezrin and moesin, and studies have shown a high concentration of radixin at cell-to-cell adherens junctions (AJ), and at cleavage furrows during cytokinesis (Tsukita et al. 1989).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      DogExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human radixin was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Peptide sequence C-QARDETKKTQND from the internal region of radixin (NP_002897.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1.03.0ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1401 detects a band of approximately 80kDa in human spleen, tonsil and lymph node cell lysates.
    • Instructions For Use

    Additional Radixin Antibody Formats

    Formats Applications Sizes available
    Radixin Antibody : Purified E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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