Western blotting analysis of human tonsil lysate (35μg protein in RIPA buffer) probed using Goat anti Human radixin antibody (AHP1401) at 0.03μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Western blotting analysis of NIH3T3 (A), HeLa (B), Jurkat (C), and A431 (D) cell line lysates (35μg protein in RIPA buffer) probed with Goat anti Human radixin antibody (AHP1401). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat anti Human Radixin antibody recognizes human radixin, a 583 amino acid inner leaf cell membrane protein bearing a single FERM domain, a member of the ezrin, radixin, moesin (ERM) protein family. Radixin is involved in cytoskeletal anchoring playing a crucial role as a linker protein between the plasma membrane and the barbed end of the actin cytoskeleton (Salles et al. 2013).
Radixin shares high sequence homology with both ezrin and moesin, and studies have shown a high concentration of radixin at cell-to-cell adherens junctions (AJ), and at cleavage furrows during cytokinesis (Tsukita et al. 1989).
Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Antiserum to human radixin was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
0.02% Sodium Azide (NaN3) 0.5% Bovine Serum Albumin
Peptide sequence C-QARDETKKTQND from the internal region of radixin (NP_002897.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP1401 detects a band of approximately 80kDa in human spleen, tonsil and lymph node cell lysates.