RAB3B Antibody | 3F12

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RAB3B Antibody | 3F12 gallery image 1

Western blot analysis of RAB3B expression in IMR-32 using preservative free Mouse anti Human RAB3 (MCA4132Z)

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RAB3B Antibody | 3F12 gallery image 2

Staining of paraffin embedded human pancreas with preservative free Mouse anti Human RAB3B (MCA4132Z)

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RAB3B Antibody | 3F12 gallery image 3

Immunofluorescence showing RAB3 in HeLa cells using preservative free Mouse anti Human RAB3 (MCA4132Z)

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  • RAB3B Antibody | 3F12 thumbnail image 1
  • RAB3B Antibody | 3F12 thumbnail image 2
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  • Mouse anti Human RAB3B:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    3F12
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4132ZIF, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4132Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human RAB3B antibody, clone 3F12 recognizes human Ras-related protein Rab-3B, also known as RAB3B, a 219 amino acid ~25 kDa lipid anchored protein likely to be involved in vesicular transport. Rab3B is enriched in inhibitory synapses in the CA1 region of the hippocampus in mice where it is involved in endocannabinoid-dependent long-term depression (Tsetsenis et al. 2011).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 120-219 of human RAB3B
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      GTPase activity
      GTP binding
      protein transport
      small GTPase mediated signal transduction
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting
      Immunohistology - Paraffin(1)0.110 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional RAB3B Antibody Formats

    Formats Clone Applications Sizes available
    RAB3B Antibody : Preservative Free 3F12 IF, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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