Pyruvate Kinase PKLR Antibody (PrecisionAbTM Antibody) | OTI1F3

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Pyruvate Kinase PKLR Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI1F3 gallery image 1

Western blot analysis of whole cell lysates probed with pyruvate kinase PKLR antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 60 second exposure. Arrow points to pyruvate kinase PKLR (molecular weight 62 kDa)

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  • Pyruvate Kinase PKLR Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI1F3 thumbnail image 1
  • Pyruvate Kinase PKLR Antibody with Control Lysate
  • Mouse anti Pyruvate Kinase PKLR
  • Pyruvate Kinase PKLR Antibody with Control Lysate
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    Pyruvate kinase PKLR antibody recognizes pyruvate kinase PKLR, also known as R-type/L-type pyruvate kinase, pyruvate kinase 1, pyruvate kinase isozyme R/L, or red cell/liver pyruvate kinase. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00264WBdatasheet pdfdatasheet pdf100 µl
      VMA00264
      VMA00264KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00264K
      VMA00264KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00264KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human pyruvate kinase PKLR antibody recognizes the pyruvate kinase PKLR, also known as R-type/L-type pyruvate kinase, pyruvate kinase 1, pyruvate kinase PKLR, pyruvate kinase isozyme R/L, pyruvate kinase isozymes L/R, pyruvate kinase isozymes R/L, pyruvate kinase type L, pyruvate kinase, liver and blood cell and red cell/liver pyruvate kinase.

        Encoded by PKLR gene, pyruvate kinase PKLR catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for PKLR (provided by RefSeq, Jul 2008).

        Mouse anti Human pyruvate kinase PKLR antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti pyruvate kinase PKLR detects a band of approximately 62 kDa in K562 cell lysates
        Anti pyruvate kinase PKLR detects a band of approximately 62 kDa in K562 cell lysates
        Anti pyruvate kinase PKLR detects a band of approximately 62 kDa in K562 cell lysates
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Mouse monoclonal antibody purified by affinity chromatography from ascites
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Full length recombinant human pyruvate kinase PKLR (NP_000289) produced in HEK293T cells
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg K562 lysate lyophilized in RIPA buffer.
        400μg K562 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        magnesium ion binding
        positive regulation of cellular metabolic process
        pyruvate kinase activity
        energy reserve metabolic process
        glycolysis
        cytosol
        potassium ion binding
        endocrine pancreas development
        ATP binding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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