PTPN6 Antibody (PrecisionAbTM Antibody) | AB01/4C6-1

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PTPN6 Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB01/4C6-1 gallery image 1

Western blot analysis of whole cell lysates probed with PTPN6 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to PTPN6 (molecular weight 64 kDa).

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  • PTPN6 Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB01/4C6-1 thumbnail image 1
  • PTPN6 Antibody with Control Lysate
  • Mouse anti PTPN6
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    PTPN6 antibody recognizes PTPN6, also known as SHP1, HCP, tyrosine-protein phosphatase non-receptor type 6 or hematopoietic cell phophatase.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00629WBdatasheet pdfdatasheet pdf100 µl
      VMA00629
      VMA00629KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00629KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human PTPN6 antibody recognizes PTPN6, also known as SHP1, HCP, tyrosine-protein phosphatase non-receptor type 6 or hematopoietic cell phophatase.

        The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported (provided by RefSeq, Jul 2008).

        Mouse anti Human PTPN6 antibody detects a band of 64 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Mouse anti PTPN6 antibody detects a band of approximately 64 kDa in HEK293 cell lysates
        Mouse anti PTPN6 antibody detects a band of approximately 64 kDa in HEK293 cell lysates
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
        Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        E. coli-derived recombinant human PTPN6 (aa 391-595)
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HEK293 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        protein binding
        nucleus
        G-protein coupled receptor protein signaling pathway
        type I interferon-mediated signaling pathway
        T cell costimulation
        interferon-gamma-mediated signaling pathway
        apoptosis
        membrane
        regulation of interferon-gamma-mediated signaling pathway
        regulation of type I interferon-mediated signaling pathway
        platelet activation
        cell junction assembly
        cytosol
        regulation of G1/S transition of mitotic cell cycle
        leukocyte migration
        protein dephosphorylation
        negative regulation of peptidyl-tyrosine phosphorylation
        positive regulation of cell proliferation
        protein tyrosine phosphatase activity
        positive regulation of phosphatidylinositol 3-kinase cascade
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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