PTF1A Antibody | 1A2

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PTF1A Antibody | 1A2 gallery image 1

Staining of paraffin-embedded human esophagus with Mouse anti Human PTF1A (MCA5119Z)

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  • Mouse anti Human PTF1A:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    1A2
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5119ZPdatasheet pdfdatasheet pdf0.1 mg
    MCA5119Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human PTF1A antibody, clone 1A2 recognizes human Pancreas transcription factor 1 subunit alpha, also known as PTF1A, Class A basic helix-loop-helix protein 29, bHLHa29, Pancreas-specific transcription factor 1a, bHLH transcription factor p48, p48 DNA-binding subunit of transcription factor PTF1 or PTF1-p48. PTF1A is a 328 amino acid transcription factor with a predicted molecular mass of ~35kDa.

      Mutations in the PTF1A gene can lead to Pancreatic and cerebellar agenesis (PACA), a disease characterized by neonatal diabetes mellitus, cerebellar agenesis, growth retardation and abnormal facial development (Sellick et al. 2004).Mutation in the PTF1A gene can also lead to a related condition Pancreatic agenesis 2 (PAGEN2), a disease characterized by pancreatic hypoplasia or agenesis, pancreatic beta cell failure resulting in neonatal insulin-dependent diabetes mellitus (Weedon et al. 2014).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 250-329 of human PTF1A
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      endocrine pancreas development
      transcription factor complex
      transcription
      tissue development
      regulation of transcription, DNA-dependent
      exocrine pancreas development
      cytoplasm
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional PTF1A Antibody Formats

    Formats Clone Applications Sizes available
    PTF1A Antibody : Preservative Free 1A2 P 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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