PTBP1 Antibody

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Jurkat cell lysate probed with Goat anti human PTBP1 N-Terminal (AHP2067)

  • PTBP1 Antibody thumbnail image 1
  • Goat anti Human PTBP1 (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2067E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human PTBP1 antibody; recognizes an epitope within the N-terminal (NT) region of human PTBP1, otherwise known as polypyrimidine tract-binding protein 1. PTPB1 is a multi-functional, ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP), implicated in pre-mRNA processing, mRNA metabolism and transport. PTPB1 contains 4 RRM motifs.

      PTBP1 is highly over-expressed in glioma and, along with its brain-specific homologue PTBP2, acts as a regulator of neural precursor cell differentiation (Zhang et al. 2013). There are 3 described isoforms of PTPB1 derived by alternative splicing, isoforms differ in having insertions between the RRM domains 2 and 3. Goat anti Human PTBP1 antibody is expected to recognize all isoforms of PTPB1.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      DogExpected from Sequence
      BovineExpected from Sequence
      MouseExpected from Sequence
      RatExpected from Sequence
      PigExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human PTBP1 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence DGIVPDIAVGTKR-C from the N-Terminal region of PTBP1 (NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      nucleotide binding
      heterogeneous nuclear ribonucleoprotein complex
      protein binding
      nuclear mRNA splicing, via spliceosome
      poly-pyrimidine tract binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1.0ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP2067 detects a band of approximately 60-65kDa in Jurkat cell lysates.
    • Instructions For Use

    Additional PTBP1 Antibody Formats

    Formats Applications Sizes available
    PTBP1 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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