PSMD14 Antibody (PrecisionAbTM Antibody)

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PSMD14 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with PSMD14 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 32 second exposure. Arrow points to PSMD14 (molecular weight 35 kDa)

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  • PSMD14 Antibody with Control Lysate
  • Rabbit anti PSMD14
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    Rabbit anti-human PSMD14 antibody recognizes the 26S proteasome non-ATPase regulatory subunit 14, also known as 26S proteasome regulatory subunit RPN11 or 26S proteasome-associated PAD1 homolog 1. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00493WBdatasheet pdfdatasheet pdf100 µl
      VPA00493
      VPA00493KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00493KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human PSMD14 antibody recognizes PSMD14 also known as 26S proteasome non-ATPase regulatory subunit 14, POH1, 26S proteasome regulatory subunit rpn11 or 26S proteasome-associated PAD1 homolog 1.

        The PSMD14 gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of PSMD14 is also located on the long arm of chromosome 2 (provided by RefSeq, Feb 2012).

        Rabbit anti Human PSMD14 antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti PSMD14 detects a band of approximately 35 kDa in K562 cell lysates
        Anti PSMD14 detects a band of approximately 35 kDa in K562 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Rabbit polyclonal antibody purified by affinity chromatography
        Rabbit polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        2% Sucrose
        0.09% Sodium Azide (NaN3)
        2% Sucrose
      • Immunogen
        Synthetic peptide directed towards the C terminal region of human PSMD14
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg K562 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        endopeptidase activator activity
        protein K63-linked deubiquitination
        regulation of proteasomal protein catabolic process
        S phase of mitotic cell cycle
        anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
        proteasome complex
        proteasome binding
        viral reproduction
        M/G1 transition of mitotic cell cycle
        ubiquitin thiolesterase activity
        positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
        DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
        metallopeptidase activity
        G1/S transition of mitotic cell cycle
        regulation of apoptosis
        regulation of cellular amino acid metabolic process
        mRNA metabolic process
        metal ion binding
        negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
        apoptosis
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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