PSMB7 Antibody (PrecisionAbTM Antibody)

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PSMB7 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with PSMB7 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 21 second exposure. Arrow points to PSMB7 (molecular weight 28 kDa)

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  • PSMB7 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • PSMB7 Antibody with Control Lysate
  • Rabbit anti PSMB7
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  • Product Type
    PrecisionAb™ Polyclonal
  • Isotype
    Polyclonal IgG
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VPA00688WBdatasheet pdfdatasheet pdf100 µl
    VPA00688
    VPA00688KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VPA00688KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
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    • Rabbit anti Human PSMB7 antibody recognizes the proteasome subunit beta type-7, also known as macropain chain Z, multicatalytic endopeptidase complex chain Z, proteasome catalytic subunit 2 or proteasome subunit Z.

      The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of PSMB7 is located on the long arm of chromosome 14 (provided by RefSeq, Jul 2012).

      Rabbit anti Human PSMB7 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Rabbit anti PSMB7 detects a band of approximately 28 kDa in MCF7 cell lysates
      Rabbit anti PSMB7 detects a band of approximately 28 kDa in MCF7 cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      20μl Rabbit polyclonal antibody purified by affinity chromatography on Protein A
      Rabbit polyclonal antibody purified by affinity chromatography on Protein A
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide
      0.09% Sodium Azide
    • Immunogen
      KLH conjugated synthetic peptide encompassing part of human PSMB7 (amino acids 180 - 211)
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg MCF7 lysate lyophilized in RIPA buffer.
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      mRNA metabolic process
      S phase of mitotic cell cycle
      apoptosis
      nucleus
      DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
      G1/S transition of mitotic cell cycle
      interspecies interaction between organisms
      threonine-type endopeptidase activity
      viral reproduction
      regulation of apoptosis
      anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
      regulation of cellular amino acid metabolic process
      negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
      cytoplasm
      M/G1 transition of mitotic cell cycle
      positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
      proteasome core complex
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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