PSMB7 Antibody (PrecisionAbTM Antibody) | 4G9

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PSMB7 Antibody (PrecisionAb<sup>TM</sup> Antibody) | 4G9 gallery image 1

Western blot analysis of whole cell lysates probed with PSMB7 antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 34 second exposure. Arrow points to PSMB7 (molecular weight 28 kDa)

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  • PSMB7 Antibody (PrecisionAb<sup>TM</sup> Antibody) | 4G9 thumbnail image 1
  • PSMB7 Antibody with Control Lysate
  • Mouse anti PSMB7
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    Mouse anti-human PSMB7 antibody recognizes proteasome subunit beta type-7, also known as macropain chain Z, multicatalytic endopeptidase complex chain Z, proteasome catalytic subunit 2 or proteasome subunit Z. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00480WBdatasheet pdfdatasheet pdf100 µl
      VMA00480
      VMA00480KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00480KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human PSMB7 antibody recognizes PSMB7 also known as proteasome subunit beta type-7, macropain chain Z, multicatalytic endopeptidase complex chain Z, proteasome catalytic subunit 2, proteasome subunit Z or proteasome subunit alpha.

        The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of PSMB7 is located on the long arm of chromosome 14 (provided by RefSeq, Jul 2012).

        Mouse anti Human PSMB7 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti PSMB7 detects a band of approximately 28 kDa in MCF7 cell lysate
        Anti PSMB7 detects a band of approximately 28 kDa in MCF7 cell lysate
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Purified IgG prepared by affinity chromatography from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Recombinant protein fragment corresponding to amino acids 58-277 of human PSMB7 produced in E. Coli
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg MCF7 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        S phase of mitotic cell cycle
        mRNA metabolic process
        anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
        positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
        G1/S transition of mitotic cell cycle
        viral reproduction
        nucleus
        interspecies interaction between organisms
        negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
        proteasome core complex
        regulation of cellular amino acid metabolic process
        DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
        M/G1 transition of mitotic cell cycle
        threonine-type endopeptidase activity
        cytoplasm
        regulation of apoptosis
        apoptosis
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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