PSMA6 Antibody (PrecisionAbTM Antibody) | 7C2

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PSMA6 Antibody (PrecisionAb<sup>TM</sup> Antibody) | 7C2 gallery image 1

Western blot analysis of whole cell lysates probed with PSMA6 antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 7 second exposure. Arrow points to PSMA6 (molecular weight 26 kDa)

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  • Mouse anti PSMA6
  • PSMA6 Antibody with Control Lysate
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    Mouse anti-human PSMA6 antibody recognizes proteasome subunit alpha type-6, also known as 27 kDa prosomal protein, PROS-27, macropain IOTA chain, multicatalytic endopeptidase complex IOTA chain or prosomal P27K protein. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00472WBdatasheet pdfdatasheet pdf100 µl
      VMA00472
      VMA00472KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00472KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human PSMA6 antibody recognizes the PSMA6, also known as 27 kDa prosomal protein, PROS-27, macropain iota chain, multicatalytic endopeptidase complex iota chain, prosomal P27K protein, proteasome iota chain, proteasome subunit alpha type-6 or proteasome subunit iota.

        The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA6 gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple transcript variants encoding several different isoforms have been found for PSMA6. A pseudogene has been identified on the Y chromosome (provided by RefSeq, Aug 2013).

        Mouse anti Human PSMA6 antibody detects a band of 26 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti PSMA6 detects a band of approximately 26 kDa in Jurkat cell lysate
        Anti PSMA6 detects a band of approximately 26 kDa in Jurkat cell lysate
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from ascites
        20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Full length recombinant protein of human PSMA6 produced in E. Coli
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        apoptosis
        regulation of inflammatory response
        threonine-type endopeptidase activity
        negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
        M/G1 transition of mitotic cell cycle
        RNA binding
        anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
        regulation of apoptosis
        G1/S transition of mitotic cell cycle
        S phase of mitotic cell cycle
        DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
        regulation of cellular amino acid metabolic process
        polysome
        mitochondrion
        proteasome core complex, alpha-subunit complex
        positive regulation of NF-kappaB transcription factor activity
        mRNA metabolic process
        NF-kappaB binding
        positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
        viral reproduction
        nuclear matrix
        sarcomere
        purine ribonucleoside triphosphate binding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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