PSMA5 Antibody (PrecisionAbTM Antibody)

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PSMA5 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with PSMA5 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 21 second exposure. Arrow points to PSMA5 (molecular weight 26 kDa)

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  • PSMA5 Antibody with Control Lysate
  • Mouse anti PSMA5
  • PSMA5 Antibody with Control Lysate
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    PSMA5 antibody recognizes proteasome subunit alpha type-5, also known as macropain subunit zeta, macropain zeta chain, multicatalytic endopeptidase complex zeta chain, proteasome component 5, proteasome subunit zeta or proteasome zeta chain. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00308WBdatasheet pdfdatasheet pdf100 µl
      VMA00308
      VMA00308KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00308K
      VMA00308KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00308KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human PSMA5 antibody recognizes the proteasome subunit alpha type-5, also known as macropain subunit zeta, macropain zeta chain, multicatalytic endopeptidase complex zeta chain, proteasome alpha 5 subunit, proteasome component 5, proteasome subunit alpha type-5, proteasome subunit zeta and proteasome zeta chain.

        The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits. Two rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. Encoded by the PSMA5 gene, proteasome subunit alpha type-5 is a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for PSMA5 (provided by RefSeq, Dec 2010).

        Mouse anti Human PSMA5 antibody detects a band of 26 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti PSMA5 detects a band of approximately 26 kDa in K562 cell lysates
        Anti PSMA5 detects a band of approximately 26 kDa in K562 cell lysates
        Anti PSMA5 detects a band of approximately 26 kDa in K562 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Purified His-tagged PSMA5 protein (fragment)
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg K562 lysate lyophilized in RIPA buffer.
        400μg K562 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        cytoplasm
        G1/S transition of mitotic cell cycle
        protein binding
        viral reproduction
        DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
        proteasome core complex, alpha-subunit complex
        regulation of cellular amino acid metabolic process
        S phase of mitotic cell cycle
        mRNA metabolic process
        positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
        M/G1 transition of mitotic cell cycle
        threonine-type endopeptidase activity
        nucleus
        negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
        apoptosis
        regulation of apoptosis
        anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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