PSMA4 Antibody (PrecisionAbTM Antibody) | OTI5D8

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PSMA4 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI5D8 gallery image 1

Western blot analysis of whole cell lysates probed with PSMA4 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to PSMA4 (molecular weight 29 kDa)

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  • PSMA4 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI5D8 thumbnail image 1
  • PSMA4 Antibody with Control Lysate
  • Mouse anti PSMA4
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  • Product Type
    PrecisionAb™ Monoclonal
  • Isotype
    IgG2b
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VMA00593WBdatasheet pdfdatasheet pdf100 µl
    VMA00593
    VMA00593KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VMA00593KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
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    • Mouse anti Human PSMA4 antibody recognizes the proteasome subunit alpha type-4, also known as macropain subunit C9, multicatalytic endopeptidase complex subunit C9, proteasome component C9, proteasome subunit HC9 or proteasome subunit L.

      The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA4 encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for PSMA4 (provided by RefSeq, Jul 2008).

      Mouse anti Human PSMA4 antibody detects a band of 29 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Anti PSMA4 detects a band of approximately 29 kDa in Jurkat cell lysates
      Anti PSMA4 detects a band of approximately 29 kDa in Jurkat cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      20μl Mouse monoclonal antibody purified by affinity chromatography Protein G from ascites
      Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine serum albumin
      <50% Glycerol
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      <50% Glycerol
    • Immunogen
      Full length HEK293T-derived recombinant protein human PSMA4
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg Jurkat lysate lyophilized in RIPA buffer
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
      identical protein binding
      interspecies interaction between organisms
      mRNA metabolic process
      G1/S transition of mitotic cell cycle
      positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
      apoptosis
      threonine-type endopeptidase activity
      DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
      viral reproduction
      proteasome core complex, alpha-subunit complex
      nucleus
      regulation of cellular amino acid metabolic process
      cytoplasm
      M/G1 transition of mitotic cell cycle
      negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
      S phase of mitotic cell cycle
      regulation of apoptosis
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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