PSIP 1 Antibody | 1C4

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PSIP 1 Antibody | 1C4 gallery image 1

Western Blot analysis of PSIP 1 expression in human kidney using Mouse anti Human PSIP 1 (MCA5064Z)

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PSIP 1 Antibody | 1C4 gallery image 2

Western blot analysis of Jurkat whole cell lysate probed with Mouse anti Human PSIP1 (MCA5064Z) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence

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  • PSIP 1 Antibody | 1C4 thumbnail image 1
  • PSIP 1 Antibody | 1C4 thumbnail image 2
  • Mouse anti Human PSIP 1:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    1C4
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5064ZWBdatasheet pdfdatasheet pdf0.1 mg
    MCA5064Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human PSIP 1 antibody, clone 1C4 recognizes human PC4 and SFRS1-interacting protein, also known as PSIP 1, CLL-associated antigen KW-7, Dense fine speckles 70 kDa protein, DFS 70, Lens epithelium-derived growth factor or Transcriptional coactivator p75/p52. PSIP 1 is a 530 amino acid, ~60 kDa transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis.

      Three isoforms of PSIP 1 generated by alternative splicing have been described. Mouse anti Human PSIP 1 antibody, clone 1C4 is expected to recognize all the described isoforms which all contain the complete unaltered immunogen sequence used to generate clone 1C4 (UniProt : O75475).

      Mutation in the PSIP1 gene is associated with pediatric acute myeloid leukemia (AML)where there may be a fusion between the NUP98 gene and the PSIP1 gene (Hussey et al. 2001).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 1-51 of human PSIP 1
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      initiation of viral infection
      interspecies interaction between organisms
      transcription
      regulation of transcription
      DNA binding
      cytosol
      nucleoplasm
      provirus integration
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional PSIP 1 Antibody Formats

    Formats Clone Applications Sizes available
    PSIP 1 Antibody : Preservative Free 1C4 WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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