Protein Kinase C Epsilon Antibody (PrecisionAbTM Antibody) | CD04/1C7

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Protein Kinase C Epsilon Antibody (PrecisionAb<sup>TM</sup> Antibody) | CD04/1C7 gallery image 1

Western blot analysis of whole cell lysates probed with protein kinase C epsilon antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to protein kinase C epsilon (molecular weight 91 kDa).

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  • Protein Kinase C Epsilon Antibody (PrecisionAb<sup>TM</sup> Antibody) | CD04/1C7 thumbnail image 1
  • Mouse anti Protein Kinase C Epsilon
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    Protein kinase C epsilon antibody recognizes PKCE, also known as Protein Kinase C Epsilon type.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2b
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00636WBdatasheet pdfdatasheet pdf100 µl
      VMA00636
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human protein kinase C epsilon antibody recognizes protein kinase C epsilon type also known PKCE.

        Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by PRKCE is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior (provided by RefSeq, Jul 2008).

        Mouse anti Human protein kinase C epsilon antibody detects a band of 91 kDa. The antibody has been extensively validated for western blotting using whole cell and tissue lysates.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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