Protein Gene Product 9.5 Antibody | BH7

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Protein Gene Product 9.5 Antibody | BH7 gallery image 1

Immunostaining of rat spinal cord section with anti-mouse PGP9.5 (red) and anti-neurofiliament H (green) antibodies

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Protein Gene Product 9.5 Antibody | BH7 gallery image 2

Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~24 kDa PGP9.5 (UCH-L1) protein

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  • Protein Gene Product 9.5 Antibody | BH7 thumbnail image 1
  • Protein Gene Product 9.5 Antibody | BH7 thumbnail image 2
  • Mouse anti Human Protein Gene Product 9.5
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    BH7
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4750GAIF, WBdatasheet pdfdatasheet pdf0.1 ml
    MCA4750GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human protein gene product 9.5 monoclonal antibody, clone BH7 recognizes protein gene product 9.5 (PGP9.5) also known as ubiquitin C terminal hydrolase 1 (UCH-L1). UCH-L1 is a thiol protease that recognises and hydrolyses a peptide bond at the C-terminal glycine of ubiquitin. This protein is closely related to UCH-L3 and both are thought to recycle ubiquitin to maintain pools of monomeric ubiquitin necessary for proteolysis. UCH-L1 is expressed in neurons and cells of the diffuse neuroendocrine system and their tumours.

      UCH-L1 and UCH-L3 have been shown to have separate and overlapping functions in the maintanence of neurons of the gracile tract, nucleus tractus solitarius (NTS) and area postrema (Kurihara et al. 2001) .
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/500
      Western Blotting1/5000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Clone BH7 detects a band of approximately 24 kDa in rat hippocampal lysate
    • Instructions For Use

    Additional Protein Gene Product 9.5 Antibody Formats

    Formats Clone Applications Sizes available
    Protein Gene Product 9.5 Antibody : Purified BH7 IF, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
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              References

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