Prolyl Hydroxylase 1 Antibody | PHD112/G7

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  • Product Type
    Monoclonal Antibody
  • Clone
    PHD112/G7
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2888GAP*, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2888GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Prolyl Hydroxylase 1 antibody, clone PHD112/G7 recognizes human prolyl hydroxylase 1 (PHD1), a 44kDa enzyme expressed abundantly in all tissues with the highest expression in testis.

      Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 1 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.

      Prolyl hydroxylase 1 might also play a role in the regulation of cell growth.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human testis
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Prolyl Hydroxylase 1 Antibody Formats

    Formats Clone Applications Sizes available
    Prolyl Hydroxylase 1 Antibody : Purified PHD112/G7 P*, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)STAR138A1 mlC, E, P, WB
      STAR138A
      Human anti Mouse IgM:FITCHCA040F0.1 mgF
      HCA040F
      Human anti Mouse IgM:HRPHCA040P0.1 mgE
      HCA040P
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Goat anti Mouse IgM:RPESTAR86PE0.5 mlF
      STAR86PE

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B

          Recommended Positive Controls

            Histology Controls

              Human testis
              • Application NameReference Images
                Western Blotting

              References

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