Mouse anti Human Prolyl Hydroxylase 1 antibody, clone PHD112/G7 recognizes human prolyl hydroxylase 1 (PHD1), a 44kDa enzyme expressed abundantly in all tissues with the highest expression in testis.
Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 1 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.
Prolyl hydroxylase 1 might also play a role in the regulation of cell growth.
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
0.09% Sodium Azide (NaN3)
Full length human PHD1 recombinantly expressed.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Paraffin(1)
This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
1. Appelhoff, R.J. et al. (2004) Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor.J Biol Chem. 279: 38458-65.
2. Boddy, J.L. et al. (2005) The androgen receptor is significantly associated with vascular endothelial growth factor and hypoxia sensing via hypoxia-inducible factors HIF-1a, HIF-2a, and the prolyl hydroxylases in human prostate cancer.Clin Cancer Res. 11: 7658-63.