Prolactin Antibody | C02-3G5

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  • Mouse anti Human Prolactin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    C02-3G5
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5967GAEdatasheet pdfdatasheet pdf0.1 mg
    MCA5967GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human prolactin, clone C02-3G5, recognizes prolactin, a peptide hormone. Prolactin is an acidic single chain polypeptide consisting of 227 amino acids and with a molecular weight of 26 kDa. Prolactin acts via stimulation of the prolactin receptor which then involves many signal transduction pathways, including JAK2/STAT, MAPK, c-src and Fyn kinase cascades (Ignacak et al. 2012).

      Prolactin is secrected by the anterior pituitary and has been primarily identified as a major stimulating factor for lactation in the postpartum period. Apart from its classical functions, prolactin affects other aspects of human body function including osmoregulation, metabolism and regulation of the immune and the central nervous system. The prolactin receptor is expressed in various tissues including mammary gland, gonads, liver, kidney, adrenal gland, brain, heart, lung, pituitary gland, uterus, skeletal muscle, skin and the immune system cells.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from ascites
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      E. coli derived recombinant protein corresponding to aa 29-227 of human prolactin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c were fused with cells of the mouse SP2/0 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • GO Terms
      regulation of multicellular organism growth
      cytosol
      hormone activity
      extracellular region
      positive regulation of JAK-STAT cascade
      cell surface receptor linked signaling pathway
      female pregnancy
      cell proliferation
      lactation
      prolactin receptor binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Prolactin Antibody Formats

    Formats Clone Applications Sizes available
    Prolactin Antibody : Purified C02-3G5 E 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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