Progesterone Receptor (pSer190) Antibody | 1154

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Progesterone Receptor (pSer190) Antibody | 1154 gallery image 1

Formalin fixed, paraffin embedded human breast cancer tissue stained with Mouse anti progesterone receptor phosphorylated at S190 antibody (MCA2406) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power)

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Progesterone Receptor (pSer190) Antibody | 1154 gallery image 2

Formalin fixed, paraffin embedded human breast cancer tissue stained with Mouse anti progesterone receptor phosphorylated at S190 antibody (MCA2406) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power)

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Progesterone Receptor (pSer190) Antibody | 1154 gallery image 3

Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of synthetic progestin agonist R5020(500 nm) showing phosphospecific immunolabeling of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform of the progesterone receptor phosphorylated at Ser190

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  • Mouse anti Progesterone Receptor (pSer190)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1154
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2406C, P *, WB*datasheet pdfdatasheet pdf0.1 ml
    MCA2406
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • This product is specific for both the PR-A and PR-B isoforms of human progesterone receptor, when phosphorylated at serine 190.

      Progesterone receptor (PR) is a member of the steroid hormone receptor superfamily, and is expressed in a range of tissues, including ovary, endometrium, uterus and placenta, and may play a key role in the regulation and differentiation of mammary glands,

      The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining , has gradually replaced ligand binding assays (LBA), to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient’s prognosis and the effectiveness of hormonal therapy.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.01%Bovine Serum Albumin
      50%Glycerol
    • Immunogen
      Synthetic phosphopeptide corresponding to an amino acid sequence within human progesterone receptor which includes phosphorylated Ser 190.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM HEPES pH7.5
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      steroid hormone receptor activity
      regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
      sequence-specific DNA binding transcription factor activity
      steroid binding
      cell-cell signaling
      cytoplasm
      receptor binding
      nucleoplasm
      enzyme binding
      zinc ion binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/1000
      Immunohistology - Paraffin(1)1/1000
      Western Blotting(2)1/1000
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose
      (2)
      For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA2406 recognises bands of approximately 90kDa and 120kDa in whole cell T47D lysates, corresponding to phospho PR-A and PR-B respectively.
    • Instructions For Use

    Additional Progesterone Receptor (pSer190) Antibody Formats

    Formats Clone Applications Sizes available
    Progesterone Receptor (pSer190) Antibody : Purified 1154 C, P *, WB* 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

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              Further Reading

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