Progesterone Receptor Antibody | 1A6

//www.bio-rad-antibodies.com/human-progesterone-receptor-antibody-1a6-mca1800.html?RenderPageSpecific=1
Progesterone Receptor Antibody | 1A6 gallery image 1

Formalin fixed, paraffin embedded breast cancer biopsy stained with Mouse anti progesterone receptor (MCA1800) followed by the Histar detection system STAR3000B following heat mediated antigen retrieval in citrate buffer pH6.0

Enlarge
Progesterone Receptor Antibody | 1A6 gallery image 2

Formalin fixed, paraffin embedded breast cancer biopsy stained with Mouse anti progesterone receptor (MCA1800) followed by the Histar detection system STAR3000B following heat mediated antigen retrieval in citrate buffer pH6.0

Enlarge
Progesterone Receptor Antibody | 1A6 gallery image 3

Formalin fixed, paraffin embedded breast cancer biopsy stained with Mouse anti progesterone receptor (MCA1800) followed by the Histar detection system STAR3000B following heat mediated antigen retrieval in citrate buffer pH6.0

Enlarge
Progesterone Receptor Antibody | 1A6 gallery image 4

Formalin fixed, paraffin embedded breast cancer biopsy stained with Mouse anti progesterone receptor (MCA1800) followed by the Histar detection system STAR3000B following heat mediated antigen retrieval in citrate buffer pH6.0

Enlarge
Progesterone Receptor Antibody | 1A6 gallery image 5

Formalin fixed, paraffin embedded breast cancer biopsy stained with Mouse anti progesterone receptor (MCA1800) followed by the Histar detection system STAR3000B following heat mediated antigen retrieval in citrate buffer pH6.0

Enlarge
Progesterone Receptor Antibody | 1A6 gallery image 6

Formalin fixed, paraffin embedded breast cancer biopsy stained with Mouse anti progesterone receptor (MCA1800) followed by the Histar detection system STAR3000B following heat mediated antigen retrieval in citrate buffer pH6.0

Enlarge
Progesterone Receptor Antibody | 1A6 gallery image 7

Formalin fixed, paraffin embedded breast cancer biopsy stained with Mouse anti progesterone receptor (MCA1800) followed by the Histar detection system STAR3000B following heat mediated antigen retrieval in citrate buffer pH6.0

Enlarge
  • Progesterone Receptor Antibody | 1A6 thumbnail image 1
  • Progesterone Receptor Antibody | 1A6 thumbnail image 2
  • Progesterone Receptor Antibody | 1A6 thumbnail image 3
  • Progesterone Receptor Antibody | 1A6 thumbnail image 4
  • Progesterone Receptor Antibody | 1A6 thumbnail image 5
  • Progesterone Receptor Antibody | 1A6 thumbnail image 6
  • Progesterone Receptor Antibody | 1A6 thumbnail image 7
  • Mouse anti Human Progesterone Receptor
  • Mouse anti Human Progesterone Receptor
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1A6
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1800TP *datasheet pdfdatasheet pdf0.1 ml
    MCA1800T
    MCA1800P *, WBdatasheet pdfdatasheet pdf1 ml
    MCA1800
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Progesterone Receptor antibody, clone 1A6 recognizes the human progesterone receptor which is a member of the steroid family of nuclear receptors. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

      The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formal fixed paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays (LBA), to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient’s prognosis and the effectiveness of hormonal therapy.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Pack Size: 1 mlTissue Culture Supernatant - lyophilised
      Pack Size: 0.1 mlTissue Culture Supernatant - liquid
      Pack Size: 1 mlTissue Culture Supernatant - lyophilised
      Pack Size: 0.1 mlTissue Culture Supernatant - liquid
    • Reconstitution
      Pack Size: 1 mlReconstitute with 1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
      Pack Size: 1 mlReconstitute with 1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
    • Preparation
    • Preservative Stabilisers
      15mMSodium Azide
      15mMSodium Azide
    • Immunogen
      Synthetic peptide corresponding to a site of predicted high antigenicity on the human progesterone receptor.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line.
    • Storage
      Pack Size: 1 mlPrior to reconstitution store at +4oC. Following reconstitution store at -20oC if preferred. This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mlStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 1 mlPrior to reconstitution store at +4oC. Following reconstitution store at -20oC if preferred. This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mlStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
      nucleoplasm
      steroid hormone receptor activity
      sequence-specific DNA binding transcription factor activity
      receptor binding
      zinc ion binding
      cytoplasm
      enzyme binding
      cell-cell signaling
      regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
      steroid binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/251/50
      Immunohistology - Paraffin(1)1/40
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/251/50
      Immunohistology - Paraffin(1)1/40
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Uterus
    • Histology Positive Control Tissue
      Uterus
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Progesterone Receptor Antibody Formats

    Formats Clone Applications Sizes available
    Progesterone Receptor Antibody : S/N 1A6 P *, WB 1 ml | 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Uterus
              Uterus

              References

              Write your review

              You may also be interested in...