350-450 mU/mg after trypsin activation. 1 U is defined as the the amount needed to hydrolyze 1 µmol of the peptide, Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg per minute.
SDS PAGE: >95%This preparation is free from gelatinase B dimers and from complexes of gelatinase B with TIMP-1 or lipocalin.
Approx. Protein Concentrations
92 kDa A singlet of 92 kDa which upon activation exhibits a relatively stable band at 84 kDa. Lower bands are observed with increased activation.
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at -20oC or for long term storage at -70oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of despatch if stored at -70oC. 3 weeks at -20oC.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.