PRKAR2A Antibody (PrecisionAbTM Antibody) | OTI4A3

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PRKAR2A Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI4A3 gallery image 1

Western blot analysis of whole cell lysates probed with PRKAR2A antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 14 second exposure. Arrow points to PRKAR2A (molecular weight 51 kDa)

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  • PRKAR2A Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI4A3 thumbnail image 1
  • Mouse anti PRKAR2A
  • PRKAR2A Antibody with Control Lysate
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    PRKAR2A antibody recognizes PRKAR2A, also known as PKR2 or protein kinase A RII-alpha subunit.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00718WBdatasheet pdfdatasheet pdf100 µl
      VMA00718
      VMA00718KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00718KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human PRKAR2A antibody recognizes PRKAR2A, also known as PKR2, cAMP-dependent protein kinase regulatory subunit RII alpha and protein kinase A RII-alpha subunit.

        cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by PRKAR2A is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER) (provided by RefSeq, Jul 2008).

        Mouse anti Human PRKAR2A antibody detects a band of 51 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Mouse anti PRKAR2A detects a band of approximately 51 kDa in HeLa cell lysates
        Mouse anti PRKAR2A detects a band of approximately 51 kDa in HeLa cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - Liquid
        Purified IgG - Liquid
      • Preparation
        Mouse monoclonal antibody affinity purified on Protein G from ascites
        20μl Mouse monoclonal antibody affinity purified on Protein G from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide
        1% Bovine Serum Albumin
        0.09% Sodium Azide
        1% Bovine Serum Albumin
      • Immunogen
        HEK293-derived full length recombinant protein of human PRKAR2A
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HeLa lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20°C, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        cytosol
        cellular response to glucagon stimulus
        cAMP-dependent protein kinase regulator activity
        regulation of insulin secretion
        regulation of protein phosphorylation
        water transport
        activation of phospholipase C activity
        intracellular signal transduction
        membrane fraction
        nerve growth factor receptor signaling pathway
        cAMP binding
        energy reserve metabolic process
        blood coagulation
        activation of protein kinase A activity
        transmembrane transport
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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