Western blotting analysis of Jurkat lysate (30μg total protein in RIPA buffer) probed with Goat anti Human PRAM1 antibody (AHP653) at 2.0μgml-1. Primary incubated for 12 hour. Signal was detected by western blot using chemiluminescence
Goat anti Human PRAM1 antibody recognises PML-RARA target gene encoding protein, which is expressed and regulated during normal human myelopoiesis.
PRAM-1 is a ~97 kDa adaptor protein, which is expressed and regulated during normal human myelopoiesis.
Purified IgG - liquid
Antisera to human PRAM1 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Bovine Serum Albumin
Peptide sequence DFCDPLENQPLPLGR corresponding to the C-terminus of PRAM1 (NP_115528).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP653 detects a band of approximately 90-100kDa in Jurkat cell lysates. An overnight incubation time of 12 hours is recommended for this antibody.
1. Moog-Lutz, C. et al. (2001) PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells.J. Biol. Chem. 276 (25) 22: 375-381