PPP2R5D Antibody (PrecisionAbTM Antibody)

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PPP2R5D Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with PPP2R5D antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 60 second exposure. Arrow points to PPP2R5D (molecular weight 75 kDa)

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  • PPP2R5D Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Goat anti PPP2R5D
  • PPP2R5D Antibody with Control Lysate
  • PPP2R5D Antibody with Control Lysate
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    PPP2R5D antibody recognizes serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform, also known as PP2A B subunit isoform B'-delta, PP2A B subunit isoform B56-delta, PP2A B subunit isoform PR61-delta or PP2A B subunit isoform R5-delta. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00028WBdatasheet pdfdatasheet pdf100 µl
      VPA00028
      VPA00028KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00028K
      VPA00028KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00028KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human PPP2R5D antibody recognizes human serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform, also known as PPP2R5D, PP2A B subunit isoform B56-delta or PP2A B subunit isoform PR61-delta. PPP2R5D is a 602 amino acid phosphatase. Three isoforms of PPP2R5D are generated by alternative splicing (Tanabe et al. 1997), all isoforms retain the C terminal region. Isoform-1 is widely expressed while isoform-2 is restricted to the brain.

        Goat anti Human PPP2R5D antibody recognizes human PPP2R5D as a band of ~75 kDa in multiple cell line lysates by western blotting under reducing conditions. The antibody also cross reacts with PPP2R5D from rodent cell line lysates tested.
      • Target Species
        Human
      • Western Blotting
        Anti PPP2R5D antibody detects a band of approximately 75 kDa in HEK293 cell lysates.
        Anti PPP2R5D antibody detects a band of approximately 75 kDa in HEK293 cell lysates.
        Anti PPP2R5D antibody detects a band of approximately 75 kDa in HEK293 cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
      • Immunogen
        Peptide sequence C-KRAEEFLTASQEAL from the C-terminal region of PPP2R5D
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HEK293 lysate lyophilized in RIPA buffer.
        400μg HEK293 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        signal transduction
        protein phosphatase type 2A complex
        nervous system development
        protein phosphatase type 2A regulator activity
        protein binding
        nucleus
        cytoplasm
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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