PPP2R2A Antibody (PrecisionAbTM Antibody)

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PPP2R2A Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with PPP2R2A antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 299 second exposure. Arrow points to PPP2R2A (molecular weight 52 kDa)

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  • PPP2R2A Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • PPP2R2A Antibody with Control Lysate
  • PPP2R2A Antibody with Control Lysate
  • Rabbit anti PPP2R2A
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    Rabbit anti human PPP2R2A antibody recognizes serine/threonine-protein phosphatase 2A regulatory subunit B alpha isoform, also known as protein phosphatase 2 (formerly 2A), regulatory subunit B (PR52), alpha isoform or serine/threonine-protein phosphatase 2A. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00328WBdatasheet pdfdatasheet pdf100 µl
      VPA00328
      VPA00328KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00328K
      VPA00328KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00328KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human PPP2R2A antibody recognizes PPP2R2A, also known as protein phosphatase 2 (formerly 2A), regulatory subunit B (PR52) alpha isoform, 55 kDa regulatory subunit B, alpha isoform and serine/threonine-protein phosphatase 2A.

        The product of the PPP2R2A gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, which associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. The PPP2R2A gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described (provided by RefSeq, Apr 2010).

        Rabbit anti Human PPP2R2A antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti PPP2R2A detects a band of approximately 52 kDa in HeLa cell lysate
        Anti PPP2R2A detects a band of approximately 52 kDa in HeLa cell lysate
        Anti PPP2R2A detects a band of approximately 52 kDa in HeLa cell lysate
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
        Rabbit polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        KLH-conjugated synthetic peptide corresponding to aa 126-160 of human PPP2R2A
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HeLa lysate lyophilized in RIPA buffer
        400μg HeLa lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        protein phosphatase type 2A complex
        protein binding
        protein phosphatase type 2A regulator activity
        protein serine/threonine phosphatase activity
        signal transduction
        protein dephosphorylation
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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