PPP2R1A Antibody (PrecisionAbTM Antibody)

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PPP2R1A Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with PPP2R1A antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc™ MP with 7 second exposure. Arrow points to PPP2R1A (molecular weight 60 kDa)

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  • Goat anti PPP2R1A
  • PPP2R1A Antibody with Control Lysate
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    PPP2R1A antibody recognizes serine/threonine-protein phosphatase 2a 65 kDa regulatory subunit a alpha isoform, also known as protein phosphatase 2, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha or medium tumor antigen-associated 61 kDa protein. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00194WBdatasheet pdfdatasheet pdf100 µl
      VPA00194
      VPA00194KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00194KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Goat anti Human PPP2R1A antibody recognizes PPP2R1A, also known as PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, medium tumor antigen-associated 61 KDa protein, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform, serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform and serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform.

        The PPP2R1A gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. PPP2R1A encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described (provided by RefSeq, Apr 2010).

        Goat anti Human PPP2R1A antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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