PPAR Alpha antibody
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Rabbit anti Human PPAR Alpha (N-Terminal)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- PPAR Alpha
- Region
- (N-TERMINAL)
Antibody Quality and Performance Guarantee
| Rabbit anti PPAR alpha antibody detects an epitope within the N-terminal region of human peroxisome proliferator-activated receptor, alpha (PPARA), a member of the nuclear hormone receptor subfamily. Upon activation, PPARA forms heterodimers with retinoid X receptors (RXRs), and functions to regulate transcription of specific gene sequences. In particular, PPARA controls genes involved in the peroxisomal and mitochondrial beta oxidation pathways, fatty acid uptake, and triglyceride catabolism in the liver and skeletal muscle. PPARA also has anti-inflammatory and anti-proliferative effects and prevents the proatherogenic effects of cholesterol accumulation in macrophages by stimulating cholesterol efflux. PPARA is the main target of fibrate drugs; hypolipidemic agents used in the treatment of dyslipidemia and the prevention of cardiovascular disease. They could also be used for the treatment of type II diabetes. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human PPAR alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- 0.02 M Potassium Phosphate
0.15 M Sodium Chloride - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to amino acids 1-18 of human PPAR alpha.
- Approx. Protein Concentrations
- IgG concentration 1.1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/8000 | 1/32000 |
| Western Blotting | |
1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1271 detects a band of approximately 52kDa in 3T3 whole cell lysate.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
Further Reading
-
Haluzík, M.M. & Haluzík, M. (2006) PPAR-alpha and insulin sensitivity.
Physiol Res. 55 (2): 115-22. -
Lefebvre, P. et al. (2006) Sorting out the roles of PPAR alpha in energy metabolism and vascular homeostasis.
J Clin Invest. 116 (3): 571-80.
- Synonyms
- PPARA
- RRID
- AB_2165752
- UniProt
- Q07869
- Entrez Gene
- PPARA
- GO Terms
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003706 ligand-regulated transcription factor activity
- GO:0003707 steroid hormone receptor activity
- GO:0005654 nucleoplasm
- GO:0008289 lipid binding
- GO:0006631 fatty acid metabolic process
- GO:0008144 drug binding
- GO:0008270 zinc ion binding
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- View More GO Terms
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010745 negative regulation of macrophage derived foam cell differentiation
- GO:0010871 negative regulation of receptor biosynthetic process
- GO:0010887 negative regulation of cholesterol storage
- GO:0010891 negative regulation of sequestering of triglyceride
- GO:0015908 fatty acid transport
- GO:0016563 transcription activator activity
- GO:0031624 ubiquitin conjugating enzyme binding
- GO:0032000 positive regulation of fatty acid beta-oxidation
- GO:0032099 negative regulation of appetite
- GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
- GO:0043565 sequence-specific DNA binding
- GO:0045820 negative regulation of glycolysis
- GO:0072363 regulation of glycolysis by positive regulation of transcription from an RNA polymerase II promoter
- GO:0072366 regulation of cellular ketone metabolic process by positive regulation of transcription from an RNA polymerase II promoter
- GO:0072369 regulation of lipid transport by positive regulation of transcription from an RNA polymerase II promoter
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