POU2F1 Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-pou2f1-antibody-vpa00130.html?RenderPageSpecific=1
POU2F1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with POU2F1 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 200 second exposure. Arrow points to POU2F1 (molecular weight 94 kDa)

Enlarge
  • POU2F1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Goat anti POU2F1
(Rated 0.0 out of 5 based on 0 customer reviews)
    POU2F1 antibody recognizes POU domain, class 2, transcription factor 1, also known as NF-A1, OTF-1, Octamer-binding transcription factor-1,Oct1, octamer-binding protein 1 or octamer-binding transcription factor 1. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00130WBdatasheet pdfdatasheet pdf100 µl
      VPA00130
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human POU2F1 antibody recognizes the POU domain, class 2, transcription factor 1, also known as NF-A1 Octamer-binding protein 1, Oct-1 Octamer-binding transcription factor 1 or OTF-1.

        The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al. 1993 (PubMed 8314572)). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT (supplied by OMIM, Jul 2010).

        Goat anti Human POU2F1 antibody detects a band of 94 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti POU2F1 detects a band of approximately 94 kDa in Ramos cell lysates.
      • Product Form
        Purified IgG - liquid
      • Preparation
        Goat polyclonal antibody purified by affinity chromatography.
      • Buffer Solution
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3) 0.5% BSA
      • Immunogen
        Peptide with the sequence C-NVQSKSNEESGDSQQ, from the internal region of the protein sequence
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        transcription repressor activity
        transcription from RNA polymerase III promoter
        sequence-specific DNA binding
        protein binding
        sequence-specific DNA binding transcription factor activity
        negative regulation of transcription, DNA-dependent
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted
                • View more products with "POU2F1" specificity.