Polyadenylate-Binding Protein 1 Antibody (PrecisionAbTM Antibody)

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Polyadenylate-Binding Protein 1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with polyadenylate-binding protein 1 antibody followed by detection with Goat anti Rabbit HRP conjugated(1/10,000,STAR208P) and visualized on the ChemiDoc MP with 26 second exposure. Arrow points to polyadenylate-binding protein 1 (molecular weight 71 kDa)

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  • Polyadenylate-Binding Protein 1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Polyadenylate-Binding Protein 1 Antibody with Control Lysate
  • Polyadenylate-Binding Protein 1 Antibody with Control Lysate
  • Rabbit anti Polyadenylate-Binding Protein 1
(Rated 0.0 out of 5 based on 0 customer reviews)
    Polyadenylate-binding protein 1 antibody recognizes polyadenylate-binding protein 1, also known as PABPC1 or poly(A) binding protein, cytoplasmic 2. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00225WBdatasheet pdfdatasheet pdf100 µl
      VPA00225
      VPA00225KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00225K
      VPA00225KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00225KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human polyadenylate-binding protein 1 antibody recognizes polyadenylate-binding protein 1, also known as poly(A) binding protein, cytoplasmic 2.

        Encoded by the PABPC1 gene, polyadenylate-binding protein 1 is a poly(A) binding protein. This protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes (provided by RefSeq, Aug 2010).

        Rabbit anti Human polyadenylate-binding protein 1 antibody detects a band of 71 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti polyadenylate-binding protein 1 detects a band of approximately 71 kDa in HeLa cell lysates
        Anti polyadenylate-binding protein 1 detects a band of approximately 71 kDa in HeLa cell lysates
        Anti polyadenylate-binding protein 1 detects a band of approximately 71 kDa in HeLa cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Pack Size: 2 Westerns20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Pack Size: 10 Westerns100μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Pack Size: 2 Westerns20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Rabbit Ig fraction prepared by ammonium sulphate precipitation
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human polyadenylate-binding protein 1
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HeLa lysate lyophilized in RIPA buffer.
        400μg HeLa lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        translation activator activity
        nucleotide binding
        spliceosomal complex
        protein C-terminus binding
        nuclear-transcribed mRNA poly(A) tail shortening
        mRNA stabilization
        cytosol
        poly(A) RNA binding
        mRNA polyadenylation
        RNA splicing
        translation
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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