Poly ADP-Ribose Antibody | 10H

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Poly ADP-Ribose Antibody | 10H gallery image 1

Published customer image:
Mouse anti Human poly ADP ribose antibody, clone 10H (MCA1480) used to illustrate PAR expression in rabbit endocardial tissue section by immunohistochemistry on formalin fixed paraffin embedded tissue sections following antigen retrieval in citrate buffer.
Image caption:
(A) Staining and quantification of nuclear PAR (poly-ADP-ribose) expression. Bar graphs depict percentage of nuclei positively stained for PAR in specimens from left ventricular epicardium, myocardium, and endocardium and right ventricle after 90 min of cardioplegia followed by 60 min of recovery. All data are given as means ± SEM. Significant differences (p<0.05) between CP- and CP+ are indicated by asterisks (*). (B) Original PAR-staining of left ventricular (LV) endocardium. Arrows indicate cell nuclei positive for PAR (stained in red).

From: Salameh A, Keller M, Dähnert I, Dhein S.
Olesoxime Inhibits Cardioplegia-Induced Ischemia/Reperfusion Injury. A Study in Langendorff-Perfused Rabbit Hearts.
Front Physiol. 2017 May 19;8:324.

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  • Poly ADP-Ribose Antibody | 10H thumbnail image 1
  • Mouse anti Human Poly(ADP-Ribose)
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  • Product Type
    Monoclonal Antibody
  • Clone
    10H
  • Isotype
    IgG3
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1480IP, P*, WBdatasheet pdfdatasheet pdf0.1 ml
    MCA1480
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Poly(Adp-Ribose) antibody, clone 10H recognizes poly (ADP-ribose) modified proteins. The antibody does not cross-react with RNA, DNA or monomers of ADP-ribose.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      BroadExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG from ascites
    • Preservative Stabilisers
      50% Glycerol
    • Immunogen
      Purified poly (ADP-ribose) polymer, 10-50 unit chain length
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation
      Western Blotting1/500
      Immunohistology - Paraffin(1)
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Poly ADP-Ribose Antibody Formats

    Formats Clone Applications Sizes available
    Poly ADP-Ribose Antibody : Purified 10H IP, P*, WB 0.1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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