POLR2A Antibody (PrecisionAbTM Antibody) | AB01/3D9

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POLR2A Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB01/3D9 gallery image 1

Western blot analysis of whole cell lysates probed with POLR2A antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, STAR207P) and visualized on the ChemiDoc MP with 26 second exposure. Arrow points to POLR2A (molecular weight 250 kDa).

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  • POLR2A Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB01/3D9 thumbnail image 1
  • Mouse anti POLR2A
  • POLR2A Antibody with Control Lysate
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    POLR2A antibody recognizes RNA Polymerase IIA, also known as DNA-directed RNA polymerase II subunit A, RPB1 or RPO2.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00613WBdatasheet pdfdatasheet pdf100 µl
      VMA00613
      VMA00613KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00613KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human POLR2A antibody recognizes RNA polymerase IIA, also known as DNA-directed RNA polymerase II subunit A, RPB1 or RPO2.

        The POLR2A gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA (provided by RefSeq, Jul 2008).

        Mouse anti Human POLR2A antibody detects a band of 250 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Mouse anti POLR2A antibody detects a band of approximately 250 kDa in K562 cell lysates
        Mouse anti POLR2A antibody detects a band of approximately 250 kDa in K562 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
        20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Synthetic peptide containing 6 repeats of the amino acid sequence YSPTSPS
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg K562 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        mRNA capping
        transcription-coupled nucleotide-excision repair
        nuclear mRNA splicing, via spliceosome
        DNA-directed RNA polymerase activity
        transcription elongation from RNA polymerase II promoter
        transcription initiation from RNA polymerase II promoter
        DNA binding
        regulation of transcription, DNA-dependent
        positive regulation of viral transcription
        metal ion binding
        DNA-directed RNA polymerase II, core complex
        ubiquitin protein ligase binding
        viral reproduction
        RNA-directed RNA polymerase activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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