PML Antibody | 1D12

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PML Antibody | 1D12 gallery image 1

Western blot analysis of PML expression in HeLa using Mouse anti Human PML:Azide Free (MCA3345Z)

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PML Antibody | 1D12 gallery image 2

Immunofluorescence showing PML in HeLa cells using Mouse anti Human PML:Azide Free (MCA3345Z)

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  • PML Antibody | 1D12 thumbnail image 1
  • PML Antibody | 1D12 thumbnail image 2
  • Mouse anti Human PML:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1D12
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3345ZIF, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA3345Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human PML antibody, clone 1D12 recognizes human protein PML, also known as PML, Promyelocytic leukemia protein, RING finger protein 71 or Tripartite motif-containing protein 19. PML is a 882 amino acid ~98 kDa protein functioning through the PML-nuclear bodies in a wide range of cellular processes including transcriptional regulation, apoptosis and the DNA damage response.

      Mutation of the PML gene has been implicated as a potential cause of acute promyelocytic leukemia (de Thé et al. 1991).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 411-510 of human PML.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional PML Antibody Formats

    Formats Clone Applications Sizes available
    PML Antibody : Preservative Free 1D12 IF, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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