Plc GAMMA-1 Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-plc-gamma-1-antibody-vma00584.html?RenderPageSpecific=1
Plc GAMMA-1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with PLC gamma-1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 7 second exposure. Arrow points to PLC gamma-1 (molecular weight 150 kDa)

Enlarge
  • Plc GAMMA-1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti Plc GAMMA-1
  • Plc GAMMA-1 Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    PrecisionAb™ Monoclonal
  • Isotype
    IgG2a
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VMA00584WBdatasheet pdfdatasheet pdf100 µl
    VMA00584
    VMA00584KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VMA00584KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
    -
    • Mouse anti Human PLC gamma-1 antibody recognizes the 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, also known as PLC-148, phosphatidylinositol phospholipase C or phosphoinositidase C.

      The protein encoded by PLCG1 gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for PLCG1 (provided by RefSeq, Jul 2008).

      Mouse anti Human PLC gamma-1 antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
    • Target Species
      Human
    • Western Blotting
      Anti PLC gamma-1 detects a band of approximately 150 kDa in Jurkat cell lysates
      Anti PLC gamma-1 detects a band of approximately 150 kDa in Jurkat cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
      20μl Mouse monoclonal antibody purified by affinity chromatography Protein G from ascites
    • Buffer Solution
      HEPES buffered saline
      HEPES buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      <50% Glycerol
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      <50% Glycerol
    • Immunogen
      E. coli-derived recombinant human PLC gamma-1
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg Jurkat lysate lyophilized in RIPA buffer
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      protein binding
      epidermal growth factor receptor signaling pathway
      interspecies interaction between organisms
      receptor signaling protein activity
      nerve growth factor receptor signaling pathway
      calcium ion binding
      cytosol
      T cell receptor signaling pathway
      positive regulation of blood vessel endothelial cell migration
      fibroblast growth factor receptor signaling pathway
      phospholipid catabolic process
      phosphatidylinositol phospholipase C activity
      axon guidance
      leukocyte migration
      blood coagulation
      plasma membrane
      positive regulation of angiogenesis
      activation of phospholipase C activity
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Write your review

              Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

              Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

              Contact Information


              Online Payment Methods:

              1. Invoice / Purchase Order
              2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
              credit cards accepted