Platelet Factor-4 antibody
Rabbit anti Human Platelet Factor-4
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Platelet Factor-4
Rabbit anti Human Platelet Factor-4 antibody recognizes platelet factor-4 (PF-4), otherwise known as CXCL4, a member of the intercrine alpha (chemokine CXC) family, released from the alpha granules of activated platelets, during platelet aggregation. PF-4 binds with high affinity to heparin, counteracting its’ anticoagulant effect, thereby promoting blood coagulation, and implicating PF-4 in wound repair. PF-4 acts as a chemotactic factor for neutrophils, monocytes and fibroblasts, and binds wih CXCR3B, a splice variant of the chemokine receptor CXCR3. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antiserum to human PF-4 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human PF-4.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.5 | 2.0ug/ml | |
Immunohistology - Paraffin 1 | |||
Western Blotting | 0.1 | 0.2ug/ml |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
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- Synonyms
- CXCL4
- RRID
- AB_2299453
- UniProt
- P02776
- Entrez Gene
- PF4
- GO Terms
- GO:0002576 platelet degranulation
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008009 chemokine activity
- GO:0008201 heparin binding
- GO:0010628 positive regulation of gene expression
- GO:0010744 positive regulation of macrophage derived foam cell differentiation
- GO:0016525 negative regulation of angiogenesis
- GO:0019221 cytokine-mediated signaling pathway
- View More GO Terms
- GO:0030168 platelet activation
- GO:0030595 leukocyte chemotaxis
- GO:0031093 platelet alpha granule lumen
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0043066 negative regulation of apoptosis
- GO:0045347 negative regulation of MHC class II biosynthetic process
- GO:0045651 positive regulation of macrophage differentiation
- GO:0045653 negative regulation of megakaryocyte differentiation
- GO:0045918 negative regulation of cytolysis
AHP1918
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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