Plasminogen Antibody

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Plasminogen Antibody gallery image 1

Published customer image:
Sheep anti Human plasminogen antibody used to detect binding of human plasminogen to the outer envelope of Francisella tularensis by immunogluorescence.
Image caption:
PLG binds to the outer envelope of FT. Laser scanning confocal microscopy of PLG-associated FTLVS. Bound huPLG ligand was detected using sheep anti-human PLG antibody followed by incubation with Dylight-488 conjugated donkey, anti-sheep/goat IgG secondary antibody. Samples were visualized using a Zeiss LSM 510 confocal microscope.

From: Clinton SR, Bina JE, Hatch TP, Whitt MA, Miller MA. Binding and activation of host plasminogen on the surface of Francisella tularensis.
BMC Microbiol. 2010 Mar 12;10:76.

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Published customer image:
Sheep anti Human plasminogen antibody used to detect binding of human plasminogen to the outer envelope of Francisella tularensis by western blotting.
Image caption:
Identification of putative PLG-binding proteins of FT. Sarkosyl-soluble and insoluble protein fractions of FTLVS were separated by SDS-PAGE and transferred to PVDF membrane. Membranes were then blotted with huPLG (3 ug/mL) followed by anti-PLG antibody and HRP-conjugated secondary antibody to detect PLG-binding proteins (Panel A). Protein bands on an identical Coomassie Blue-stained SDS-PAGE gel corresponding to those identified via blotting (Panel B) were excised and identified using proteomic methodologies (Panel C).
BMC Microbiol. 2010 Mar 12;10:76.

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Sheep anti Human plasminogen antibody used for the detection of plasminogen in Jurkat cell lysates by western blotting.
Image caption:
Necrotic cells harbour misfolded proteins and generate surface-bound plasmin which increases their phagocytosis by human MoDCs. (A) Uninjured and necrotic Jurkat lymphocytes were stained with 10 mg/L 7AAD and 10 mg /L Thiazine Red for 15 min then subjected to flow cytometry. (B) The Triton-insoluble fractions of uninjured and necrotic Jurkat lymphocytes were subjected to SDS-PAGE under reducing conditions and subsequent Coomassie staining. (C) Uninjured and necrotic Jurkat lymphocytes were incubated with t-PA and/or plasminogen for 15 min and washed. Total cellular protein lysates were prepared and subjected to SDS-PAGE under reducing conditions and subsequent immunoblot analysis. Most of the t-PA used was in single-chain form (~70 kDa), however a small extent of two-chain t-PA was also apparent (the band indicated by the asterisk at ~35 kDa). (D) PKH67-labelled necrotic Jurkat lymphocytes were treated with the indicated reagents for 15 min, then washed and incubated with PKH26-labelled human MoDCs. 24 h later, the proportion of double-positive (PKH67positive and PKH26positive) MoDCs was assessed by flow cytometry. Data are displayed as fold-change in double-positive MoDCs (mean ± s.e.m.; n = 3–5 independent experiments). Data was normalized to the group where necrotic cells received no exogenous reagent. **p<0.01 and ***p<0.001 by 1-way ANOVA with Newman-Keuls post-hoc analysis.

From: Borg RJ, Samson AL, Au AE-L, Scholzen A, Fuchsberger M, Kong YY, et al. (2015) Dendritic Cell-Mediated Phagocytosis but Not Immune Activation Is Enhanced by Plasmin.
PLoS ONE 10(7): e0131216.

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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    7440-0104FIFdatasheet pdfdatasheet pdf1 ml
    7440-0104F
    7440-0104PC, Edatasheet pdfdatasheet pdf1 ml
    7440-0104P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti Human plasminogen antibody recognizes human plasminogen, a ~90 kDa single-chain glycoprotein and inactive precursor (zymogen) of plasmin, secreted by the liver and present in the blood and majority of extravascular body fluids.

      Plasminogen is the central component in the fibrinolytic system, and is converted into active plasmin through cleavage by the endogenous activators urokinase (u-PA) and tissue plasminogen activator (t-PA), in the presence of fibrin. The level of plasminogen is maintained in the plasma at around 200mg/L, with an increase being associated with hypothyroidism, obesity and pregnancy, and deficiencies with many disorders including thrombosis, liver disease, sepsis and hyperthyroidism.

      Sheep anti Human plasminogen antibody has not been cross adsorbed and may cross react with plasminogen from other species.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Catyes
      Ratyes
      Dogyes
      Bovineyes
      Guinea Pigyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human plasminogen were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.01%Thiomersal
    • Immunogen
      Native, highly purified.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      extracellular space
      fibrinolysis
      platelet activation
      extracellular matrix disassembly
      negative regulation of cell proliferation
      extrinsic to external side of plasma membrane
      tissue remodeling
      platelet alpha granule lumen
      positive regulation of fibrinolysis
      apolipoprotein binding
      platelet degranulation
      negative regulation of fibrinolysis
      serine-type endopeptidase activity
      negative regulation of cell-substrate adhesion
      cell surface binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Plasminogen Antibody Formats

    Formats Applications Sizes available
    Plasminogen Antibody : FITC IF 1 ml
    Plasminogen Antibody : HRP C, E 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence
                Western Blotting

              References

              Further Reading

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