Plakophilin 1 Antibody (PrecisionAbTM Antibody)

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Plakophilin 1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with plakophilin 1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 25 second exposure. Arrow points to plakophilin 1 (molecular weight 75 kDa)

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  • Plakophilin 1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Plakophilin 1 Antibody with Control Lysate
  • Mouse anti Plakophilin 1
  • Plakophilin 1 Antibody with Control Lysate
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    Plakophilin-1 antibody recognizes plakophilin-1, also known as band 6 protein or PKP1. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00096WBdatasheet pdfdatasheet pdf100 µl
      VMA00096
      VMA00096KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00096K
      VMA00096KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00096KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human plakophilin 1 recognizes a member of the plakophilin family which have been identified as junctional proteins that are essential for the formation and stabilization of desmosomal cell contacts. Plakophilin 1 is a protein that in humans is encoded by the PKP1 gene, with a molecular weight of 75 kDa. This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families.

        Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may be involved in molecular recruitment and stabilization during desmosome formation and in the formation of desmosomal plaques where it is associated with desmoplakin. It is not found in cell types that have non-epithelial desmosomes and is absent in fibroblasts and other connective tissue types, including sarcomas. Mutations in this gene have been associated with the ectodermal dysplasia/skin fragility syndrome.

        Mouse anti Human plakophilin 1 antibodu has been found to be specific for the armadillo repeat domain of plakophilin 1. This antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti plakophilin 1 antibody detects a band at 75 kDa in A431 cell lysates
        Anti plakophilin 1 antibody detects a band at 75 kDa in A431 cell lysates
        Anti plakophilin 1 antibody detects a band at 75 kDa in A431 cell lysates
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Recombinant fusion protein fragment, consisting of amino acids 235-726 of human plakophilin 1, fused to maltose binding protein.
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg A431 lysate lyophilized in RIPA buffer.
        400μg A431 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store at -20oC only.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        structural constituent of epidermis
        desmosome
        signal transducer activity
        intermediate filament binding
        cell adhesion
        nucleus
        cellular component disassembly involved in apoptosis
        intermediate filament
        multicellular organismal development
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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