Placental Alkaline Phosphatase Antibody | H17E2

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Published customer image:
Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 used for the detection of PLAP reporter expression on peripheral blood granulocytes by flow cytometrry.
Image caption:
Figure 3. Activation of the Cre-reporter gene is less efficient in Z/AP and Z/EG mice than in R26R-EYFP mice. All reporter strains were crossed to the general deleter strain, pCX-NLS-Cre, and activation of each reporter gene was assessed by flow cytometry of peripheral blood leukocytes at 12 weeks of age (A). All reporter strains were also crossed to the myeloid-specific LysM-Cre strain, and activation of each reporter gene was assessed by flow cytometry of peripheral blood granulocytes at 12 weeks of age (B). Representative histograms demonstrating reporter expression in peripheral blood granulocytes are shown in (C–E) for LysM-Cre/R26R-EYFP, LysM-Cre/ZAP and LysM-Cre/Z/EG mice respectively (solid lines). Reporter expression in BL/6 mice is shown in dotted lines.

From: Long MA, Rossi FMV (2009) Silencing Inhibits Cre-Mediated Recombination of the Z/AP and Z/EG Reporters in Adult Cells. PLoS ONE 4(5): e5435.

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  • Mouse anti Human Placental Alkaline Phosphatase
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  • Product Type
    Monoclonal Antibody
  • Clone
    H17E2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2091C *, E, Fdatasheet pdfdatasheet pdf0.2 mg
    MCA2091
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 recognizes human placental alkaline phosphatase, also known as Alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type or Placental alkaline phosphatase 1. Placental alkaline phosphatase is a 535 amino acid ~67 kDa GPI-anchored transmembrane glycoprotein bearing a 22 amino acid signal peptide and a 29 amino acid pro-peptide region, cleaved to produce the mature form. Placental alkaline phosphatase is expressed in trophoblast cells of normal human placenta (Travers et al. 1984), in seminomas (Lange et al. 1982)and in ovarian carcinoma (Tholander et al. 1990).

      Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 has been widely used in immunolocalization studies of germ cell tumours and may also be used for the measurement of soluble placental alkaline phosphatase by ELISA.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Placental membrane.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      alkaline phosphatase activity
      integral to membrane
      anchored to membrane
      cell surface
      metal ion binding
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry10ug/ml
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Placenta, seminoma
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Placental Alkaline Phosphatase Antibody Formats

    Formats Clone Applications Sizes available
    Placental Alkaline Phosphatase Antibody : Purified H17E2 C *, E, F 0.2 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Placenta, seminoma
              • Application NameReference Images
                Flow Cytometry

              References

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