PINK1 Antibody | 11408

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PINK1 Antibody | 11408 gallery image 1

Paraffin embedded human testis stained with Human anti Human PINK1 (HCA150) and mouse anti strep-tag (MCA2488) followed by Histar detection (STAR3000A)

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  • PINK1 Antibody | 11408 thumbnail image 1
  • Human anti Human PINK1
  • Human anti Human PINK1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    11408
  • Isotype
    HuCAL Fab monovalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA150C, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA150
    HCA150AC, P *, WBdatasheet pdfdatasheet pdf50 µg
    HCA150A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Human PINK1 antibody, clone 11408 recognizes Human PTEN-induced putative Kinase protein 1 (PINK1), a 581 aa single pass membrane protein belonging the Serine/Threonine protein kinase.

      PINK1 protects against mitochondrial dysfunction during cellular stress, possibly by phosphorylating mitochondrial proteins. PINK1 is highly expressed in tissues such as the heart, skeletal muscle and testis. Lower expression levels are detected in the brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine.

      Defects in PINK1 are the cause of autosomal recessive early-onset Parkinson disease 6 (PARK6).
    • Intended Use
    • Target Species
      Human
    • Product Form
      A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli.. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli.. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Peptide corresponding aa 321 – 511 of Human PINK1.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      intracellular protein kinase cascade
      protein serine/threonine kinase activity
      mitochondrion degradation
      mitochondrial outer membrane
      integral to membrane
      cell death
      peptidyl-serine phosphorylation
      magnesium ion binding
      positive regulation of release of cytochrome c from mitochondria
      positive regulation of I-kappaB kinase/NF-kappaB cascade
      regulation of protein complex assembly
      calcium-dependent protein kinase activity
      cytosol
      ubiquitin protein ligase binding
      C3HC4-type RING finger domain binding
      regulation of protein ubiquitination
      response to stress
      ATP binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1:50
      Western Blotting2ug/ml
      Immunohistology - Paraffin(1)1:50
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1:50
      Western Blotting2ug/ml
      Immunohistology - Paraffin(1)1:50
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human testis
    • Histology Positive Control Tissue
      Human testis
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      HCA150A detects a band of approximately 70kDa In human brain cell lysates.
    • Western Blotting
      HCA150 detects a band of approximately 70kDa In human brain cell lysates.
    • Instructions For Use
    • Instructions For Use

    Additional PINK1 Antibody Formats

    Formats Clone Applications Sizes available
    PINK1 Antibody : Purified 11408 C, P *, WB 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti V5-Tag:Alk. Phos.MCA1360A0.1 mgE, WB
      MCA1360A
      Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
      MCA2489P
      Mouse anti V5-TagMCA13601 mgC, E, F, IF, IP, R, WB
      MCA1360
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti V5-Tag:Alk. Phos.MCA1360A0.1 mgE, WB
      MCA1360A
      Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
      MCA2489P
      Mouse anti V5-TagMCA13601 mgC, E, F, IF, IP, R, WB
      MCA1360

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B

          Recommended Positive Controls

            Histology Controls

              Human testis
              Human testis

              References

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