PI-4 Kinase 2A Antibody (PrecisionAbTM Antibody)

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PI-4 Kinase 2A Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with PI-4 kinase 2A antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 81 second exposure. Arrow points to PI-4 kinase 2A (molecular weight 54 kDa)

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  • PI-4 Kinase 2A Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • PI-4 Kinase 2A Antibody with Control Lysate
  • Rabbit anti PI-4 Kinase 2A
  • PI-4 Kinase 2A Antibody with Control Lysate
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    Rabbit anti human PI4K2A antibody recognizes phosphatidylinositol 4-kinase type 2-alpha, also known as phosphatidylinositol 4-kinase type II (PI4KII) or phosphatidylinositol 4-kinase type 2A. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00327WBdatasheet pdfdatasheet pdf100 µl
      VPA00327
      VPA00327KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00327K
      VPA00327KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00327KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human PI-4 kinase 2A antibody recognizes PI-4 kinase 2A, also known as phosphatidylinositol 4-kinase type II (PI4KII), phosphatidylinositol 4-kinase type 2A and phosphatidylinositol 4-kinase type II-alpha.

        Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI-4 Kinase 2A phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al. 2001 PubMed 11244087, OMIM, Mar 2008).

        Rabbit anti Human PI-4 kinase 2A antibody detects a band of 54 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti PI-4 kinase 2A detects a band of approximately 54 kDa in Jurkat cell lysate
        Anti PI-4 kinase 2A detects a band of approximately 54 kDa in Jurkat cell lysate
        Anti PI-4 kinase 2A detects a band of approximately 54 kDa in Jurkat cell lysate
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
        Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
      • Immunogen
        KLH-conjugated synthetic peptide corresponding to aa 387-415 of human PI-4 kinase 2A
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer
        400μg Jurkat lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        cytoplasm
        integral to plasma membrane
        ATP binding
        1-phosphatidylinositol 4-kinase activity
        membrane raft
        phosphatidylinositol biosynthetic process
        magnesium ion binding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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