PI-3 Kinase C2 Subunit Alpha Antibody (PrecisionAbTM Antibody) | OTI1B8

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PI-3 Kinase C2 Subunit Alpha Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI1B8 gallery image 1

Western blot analysis of whole cell lysates probed with PI-3 kinase C2 subunit alpha antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 68 second exposure. Arrow points to PI-3 kinase C2 subunit alpha (molecular weight 191 kDa)

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  • PI-3 Kinase C2 Subunit Alpha Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI1B8 thumbnail image 1
  • Mouse anti PI-3 Kinase C2 Subunit Alpha
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    PI-3 kinase C2 subunit alpha antibody recognizes phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, also known as C2-containing phosphatidylinositol kinase or PI3K-C2-alpha. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2b
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00263WBdatasheet pdfdatasheet pdf100 µl
      VMA00263
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human PI-3 kinase C2 subunit alpha antibody recognizes the phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, also known as C2-containing phosphatidylinositol kinase, PI3K-C2-alpha, PI3K-C2alpha, phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha, phosphoinositide 3-kinase-C2-alpha, phosphoinositide-3-kinase, class 2, alpha polypeptide and ptdIns-3-kinase C2 subunit alpha.

        The protein encoded by PIK3C2A gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. (provided by RefSeq, Jul 2008).

        Mouse anti Human PI-3 kinase C2 subunit alpha antibody detects a band of 191 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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