Phosphodiesterase 2A Antibody

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Staining of formalin fixed, paraffin embedded human kidney with Rabbit anti Human phosphodiesterase 2A (AHP1147)

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  • Rabbit anti Human Phosphodiesterase 2A (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1147P *datasheet pdfdatasheet pdf50 µg
    AHP1147
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti human phosphodiesterase 2A (N-terminal) antibody detects an epitope within the N-terminal (NT) region of phosphodiesterase 2A (PDE2A), a 100kD cyclic nucleotide phosphodiesterase (PDE). Three isoforms of PDE2A have been reported (PDE2A1, PDE2A2, and PDE2A3) which arise from a single gene by alternative splicing events. PDE2A can degrade both cAMP and cGMP, but degrades cGMP with greater efficacy.

      PDE2A is expressed in a variety of tissue types, with greatest expression in the brain, where it is localised in the cerebellum and neocortex. PDE2A has been implicated in penile erectile dysfunction and in the regulation of fluid and inflammatory cell transport across the endothelial cell barrier.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseno
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to phosphodiesterase 2A (NT) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide: N-terminal region of human phosphodiesterase 2A conjugated to KLH.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      metal ion binding
      3',5'-cyclic-AMP phosphodiesterase activity
      cGMP-stimulated cyclic-nucleotide phosphodiesterase activity
      protein binding
      3',5'-cyclic-GMP phosphodiesterase activity
      signal transduction
      cGMP binding
      membrane
      cytosol
      platelet activation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin(1)1525ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Kidney
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Phosphodiesterase 2A Antibody Formats

    Formats Applications Sizes available
    Phosphodiesterase 2A Antibody : Purified P * 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          Histar Detection SystemSTAR3000C500 TestsC, P
          STAR3000C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Kidney

              References

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