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Phosphodiesterase 1C Antibody

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Staining of formalin fixed, paraffin embedded human thyroid with Rabbit anti Human phosphodiesterase 1C (AHP1145)

  • Phosphodiesterase 1C Antibody thumbnail image 1
  • Rabbit anti Human Phosphodiesterase 1C (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1145P *datasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human Phosphodiesterase 1C antibody detects an epitope within the C-terminal (CT) region of phosphodiesterase 1C (PDE1C), a subtype of the PDE1 enzyme. PDE1C is activated by calmodulin binding in the presence of calcium, and degrades the cyclic nucleotides cAMP and cGMP. A number of PDE1C isoforms exist, each with different affinities for cAMP and cGMP. They have a range of molecular weights from ~65-70 kDa.

      PDE1C is a major regulator of smooth muscle cell (SMC) proliferation in humans, and plays an important role in the development of organs containing smooth muscle. PDE1C may also have a role in olfaction, regulation of sperm function and neuronal signaling.
      Inhibition of PDE1C may prove beneficial in the treatment of diseases such as atherosclerosis and pulmonary hypertension where excessive SMC proliferation occurs.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to phosphodiesterase 1C (CT) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.01% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide corresponding to the C-terminal domain of human phosphodiesterase 1C conjugated to KLH.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      calmodulin-dependent cyclic-nucleotide phosphodiesterase activity
      calmodulin binding
      nerve growth factor receptor signaling pathway
      activation of phospholipase C activity
      metal ion binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin(1)210ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Phosphodiesterase 1C Antibody Formats

    Formats Applications Sizes available
    Phosphodiesterase 1C Antibody : Purified P * 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Histar Detection SystemSTAR3000B150 TestsC, P
          Histar Detection SystemSTAR3000A50 TestsC, P
          Histar Detection SystemSTAR3000C500 TestsC, P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Phosphodiesterase 1C Antibody thumbnail image 1


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