Phenylalanine-4-Hydroxylase Antibody | 12257

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Phenylalanine-4-Hydroxylase Antibody | 12257 gallery image 1

COS7 lysate (non-transfected, no PAH, A); A549 lysate (non-transfected, no PAH, B); HEPG2 lysate (non-transfected, endogenous PAH, C); HEK293 lysate (PAH transfected,non stable PAH mutant, D); HEK293 lysate (PAH transfected, wild type PAH, E); Jurkat lysate (non-transfected, no PAH, F) and COS7 lysate (PAH transfected, wild type PAH, G) probed with Human anti Phenylalanine-4-Hydroxylase (HCA141). Image kindly provided by Dr. med. Søren W. Gersting, Ludwig-Maximilians-University Munich

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  • Human anti Human Phenylalanine-4-Hydroxylase
  • Human anti Human Phenylalanine-4-Hydroxylase
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    12257
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA141WBdatasheet pdfdatasheet pdf0.1 mg
    HCA141
    HCA141AWBdatasheet pdfdatasheet pdf50 µg
    HCA141A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Human Phenylalanine-4-Hydroxylase antibody, clone 12257 recognizes human Phenylalanine-4-Hydroxylase (PAH), a 51kDa homotetrameric protein expressed abundantly in the liver.

      PAH is an essential enzyme in the metabolic pathway where it is responsible for removing excess phenylalanine by converting it to tyrosine. Defects in the PAH gene, which reduce PAH activity, lead to phenylketonuria (PKU) , a disorder resulting in mental retardation.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Pack Size: 0.1 mg A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      Pack Size: 50 µg A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied as liquid.
      Pack Size: 0.1 mg A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      Pack Size: 50 µg A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied as liquid.
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
    • Preparation
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
    • Preservative Stabilisers
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      Pack Size: 50 µg0.01% Thiomersal
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      Pack Size: 50 µg0.01% Thiomersal
    • Immunogen
      Recombinant human Phenylalanine-4-Hydroxylase fusion protein with MBP
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.1 mgTotal protein concentration 0.1mg/ml
      Pack Size: 50 µg0.5 mg/ml
      Pack Size: 0.1 mgTotal protein concentration 0.1mg/ml
      Pack Size: 50 µg0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Pack Size: 0.1 mgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
      oxidation-reduction process
      L-phenylalanine catabolic process
      catecholamine biosynthetic process
      phenylalanine 4-monooxygenase activity
      neurotransmitter biosynthetic process
      cytosol
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Phenylalanine-4-Hydroxylase Antibody Formats

    Formats Clone Applications Sizes available
    Phenylalanine-4-Hydroxylase Antibody : Purified 12257 WB 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:Alexa Fluor® 488MCA2200A488100 Tests/1mlF *
      MCA2200A488
      Mouse anti c-Myc:Alexa Fluor® 647MCA2200A647100 Tests/1mlF *
      MCA2200A647
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti c-Myc:FITCMCA2200F0.1 mgF *
      MCA2200F
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
      MCA2489P
      Mouse anti c-Myc:RPEMCA2200PE100 TestsWB
      MCA2200PE
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:Alexa Fluor® 488MCA2200A488100 Tests/1mlF *
      MCA2200A488
      Mouse anti c-Myc:Alexa Fluor® 647MCA2200A647100 Tests/1mlF *
      MCA2200A647
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti c-Myc:FITCMCA2200F0.1 mgF *
      MCA2200F
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
      MCA2489P
      Mouse anti c-Myc:RPEMCA2200PE100 TestsWB
      MCA2200PE

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          HuCAL Fab-dHLX-MSx2 Negative ControlHCA102A50 µgC, E, F, P, WB
          HCA102A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          HuCAL Fab-dHLX-MSx2 Negative ControlHCA1020.1 mgC, E, F, P, WB
          HCA102

          Recommended Positive Controls

            Histology Controls

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