PGA3 Antibody | AbD10785
Pepsin antigen detected with Human anti Human PGA3 (HCA196) followed by Mouse anti c-myc:HRP (MCA2200P)
Enlarge- Human anti Human PGA3
-
Product TypeMonoclonal Antibody
-
CloneAbD10785
-
IsotypeHuCAL Fab bivalent
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
-
- Human anti Human PGA3 antibody, clone AbD10785 recognizes human PGA3, also known as Pepsin A-3 and Pepsinogen-3, a secreted enzyme belonging to the peptidase A1 family.
PGA3 is secreted by the gastric mucosa of mammals.
-
Intended Use
-
Target SpeciesHuman
-
Product FormA bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
-
Reconstitution
-
PreparationMetal chelate affinity chromatography
-
Preservative Stabilisers0.01% Thiomersal
-
ImmunogenPepsin N1 fusion protein
-
Purity
-
Approx. Protein ConcentrationsTotal protein concentration 0.5 mg/ml
-
Reagents In The Kit
-
Preparing The Antibody
-
Test Principle
-
Buffer SolutionPhosphate buffered saline
-
StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life12 months from date of despatch
-
AcknowledgementsSold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. -
Licensed UseFor in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
-
RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA Western Blotting - Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical AdviceRecommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Recommended Protocol
- ELISAThis product is suitable for use in indirect ELISA applications
- Immunohistology
- Histology Positive Control Tissue
- Immunofluorescence
- Western BlottingClone AbD10785 detects a band of approximately 47 kDa from human gastric fluid samples
- Instructions For Use
Additional PGA3 Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| PGA3 Antibody : Purified | AbD10785 | E, WB | 0.1 mg |
- Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | 0.1 mg | E, WB | MCA1396A | |
| Mouse anti c-Myc:Alk.Phos. | MCA2200A | 0.1 mg | C, E, P, WB* | MCA2200A | |
| Mouse anti Histidine Tag:Biotin | MCA1396B | 0.1 mg | E, WB | MCA1396B | |
| Mouse anti c-Myc:Biotin | MCA2200B | 0.1 mg | C, E, P, WB* | MCA2200B | |
| Mouse anti c-Myc:HRP | MCA2200P | 0.1 mg | C, E, P, WB* | MCA2200P | |
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | 125 µl | E, WB | MCA5995P |
