Peroxiredoxin 6 Antibody (PrecisionAbTM Antibody)

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Peroxiredoxin 6 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with peroxiredoxin 6 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 5 second exposure. Arrow points to peroxiredoxin 6 (molecular weight 25 kDa)

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  • Peroxiredoxin 6 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Peroxiredoxin 6 Antibody with Control Lysate
  • Mouse anti Peroxiredoxin 6
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  • Product Type
    PrecisionAb™ Monoclonal
  • Isotype
    IgG2b
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VMA00583WBdatasheet pdfdatasheet pdf100 µl
    VMA00583
    VMA00583KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VMA00583KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
    -
    • Mouse anti Human peroxiredoxin 6 antibody recognizes peroxiredoxin 6, also known as 1-Cys PRX, 1-Cys peroxiredoxin, acidic calcium-independent phospholipase A2, PRDX6 or non-selenium glutathione peroxidase.

      The protein encoded by PRDX6 gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury (provided by RefSeq, Jul 2008).

      Mouse anti Human peroxiredoxin 6 antibody detects a band of 25 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
    • Target Species
      Human
    • Western Blotting
      Anti peroxiredoxin 6 detects a band of approximately 25 kDa in HeLa cell lysates
      Anti peroxiredoxin 6 detects a band of approximately 25 kDa in HeLa cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      20μl Mouse monoclonal antibody purified by affinity chromatography Protein G from ascites
      Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
    • Buffer Solution
      HEPES buffered saline
      HEPES buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      <50% Glycerol
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      <50% Glycerol
    • Immunogen
      E. coli-derived recombinant human peroxiredoxin 6
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg HeLa lysate lyophilized in RIPA buffer
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      protein binding
      lysosome
      cell redox homeostasis
      peroxiredoxin activity
      phospholipase A2 activity
      cytoplasmic membrane-bounded vesicle
      phospholipid catabolic process
      oxidation-reduction process
      cytosol
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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