Peroxiredoxin 4 Antibody | 3A1

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Peroxiredoxin 4 Antibody | 3A1 gallery image 1

Mouse anti peroxiredoxin 4 recognizes a band of approximately 28 kDa in HeLa cell lysate

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Peroxiredoxin 4 Antibody | 3A1 gallery image 2

ICC staining of HeLa cells with anti peroxiredoxin 4 antibody

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  • Peroxiredoxin 4 Antibody | 3A1 thumbnail image 1
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  • Mouse anti Peroxiredoxin 4
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  • Product Type
    Monoclonal Antibody
  • Clone
    3A1
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6025IC, IP, WBdatasheet pdfdatasheet pdf50 µg
    MCA6025
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti peroxiredoxin 4 antibody, clone 3A1 recognizes peroxiredoxin 4 also known as antioxidant enzyme AOE372 and thioredoxin peroxidase AO372. Peroxiredoxin 4 regulates the activation of NFkappaB in the cytoplasm through the modulation of Iκbα phosphorylation (Jin et al. 1997).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      IgG fraction prepared by ammonium sulphate precipitation
    • Preservative Stabilisers
      0.03% Sodium Azide (NaN3)
      0.01% Bovine Serum Albumin
      50% Glycerol
    • Immunogen
      Recombinant human fragment protein (without secretion leader sequence) purified from E.coli
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      HEPES buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      thioredoxin peroxidase activity
      I-kappaB phosphorylation
      oxidation-reduction process
      cell redox homeostasis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunocytochemistry20 ul/ml
      Immunoprecipitation1.02.0 ul/ml
      Western Blotting2.04.0 ul/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Mouse anti peroxiredoxin 4 recognizes a band of approximately 28 kDa in HeLa cell lysate
    • Instructions For Use

    Additional Peroxiredoxin 4 Antibody Formats

    Formats Clone Applications Sizes available
    Peroxiredoxin 4 Antibody : Purified 3A1 IC, IP, WB 50 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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